Autoradiography At the Cellular Level. Physical Techniques by Brigitte Schultze

By Brigitte Schultze

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Latent images produced in the wet emulsion are erased immediately by fading. In thick emulsions, tracks of β- or α-emitters can be followed to their origin in the specimen. The time for developing and fixing is con­ siderably longer than for the thin emulsions. A modification of the liquid emulsion technique was introduced by 32 King et al. (1951), who added P-labeled Paramecium cultures to liquid Ilford G-5 emulsion. The intimate contact between specimen and emul­ sion permits better distinction between the tracks of the specimen, which are emitted in all directions, and the background tracks from contamina­ tion of the emulsion with a-emitters.

Dry-mounting, preferably in a low-humidity darkroom, of unfixed, unembedded freeze-dried sections on emulsion-coated slides (Kodak NTB-3) previously stored over Drierite. 5. Exposure in a freezer at — 15°C. 6. Photographic development with staining as final step. With this method and special procedures to make the sections adhere to the emulsion during the photographic process, tissue preservation and autoradiographic resolution comparable to that obtained with standard methods were achieved. VI.

Wimber (1963), Wimber and Quastler (1963), and Davies and Wimber (1963) used a double coating of Kodak N T B liquid emulsion by the dip­ ping technique, where the slides with the first emulsion layer were allowed to dry for 30 min prior to the application of the second emulsion. Lennartz et al. (1964) and Pilgrim et al. (1966) working simultaneously 3 14 with H - and C-labeled thymidine used a thin stripping film (5 μ thick without gelatin base, experimental scientific plates V 1062, unbacked) for the first layer and liquid emulsion Ilford G-5 for the second layer (ca.

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