By Cecelia M O'Connor; G E W Wolstenholme; International Union of Biological Sciences
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We had earlier described the existence of such a transferase in galactoseadapted yeast (Kalckar, Braganca and Munch-Petersen, 1953; Munch-Petersen, Kalckar and Smith, 1955). This uridyl transferase catalyses a reaction between PGal and uridine triphosphate (URPPP) according to the equation : PGal .. + URPPP . + PP . , 1957). Moreover, Isselbacher (1957) found the alternative transferase in rat liver. Hence, it is quite conceivable that the alternative transferase is present in small amounts in human liver and may increase in adults.
Arch. , 61, 88. MAXWELL, E. S. (1957). J . biol. , 229, 139. , KALCKAR, H. , and BURTOX,R. M. (1955). Biockim. biophys. Acta, 18, 444. MORSE,M. , LEDERBERG, E. ,and LEDERBERG, J. (1956). Genetics, 41, 143, 758. , KALCKAR, H. , and SMITH,E. E. B. (1955). Biol. Medd. ), 22, 3. NAJJAR,V. (1959). , Bern, 1958. DISCUSSION 36 NEUFELD, E. , PUTRIAN, E. , and HASSID,W. Z. (1957). Arch. , 69, 602. , KOMROWER, G. , and HOLZEL, A. (1956). Biochem. J . , 62, 34. SIDBURY, J. B. (1957). Abstr. Proc. Biol. , Amer.
Gunn’s patients. The new spectrophotometric method gave results on those two patients which were not in agreement with results produced by accepted standard methods. Furthermore, the spectrophotometer permitted the observation of progress curves of a reaction catalysed by cholinesterase (Kalow, Genest and Staron, 1956); these progress curves were of a peculiar shape when the sera of Dr. Gunn’s patients served as the source of enzyme. These observations started the investigations which are to be reviewed here.