By Meral Beksac
This quantity is a compendium of state of the art molecular tools for the winning transplantation of hematopoietic stem cells. The participants are world-renown leaders within the box. They describe promising instruments for stem cellphone transplant study versions, equivalent to in vivo bioluminescence imaging. They talk about HLA typing, PCR-SSP typing, and HLA antigens. This quantity is a useful resource for biochemists, molecular biologists, and clinicians.
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Additional info for Bone Marrow and Stem Stell Transplantation (Methods in Molecular Medicine)
No. 107689-100G). Stock concentration 5 mg/mL in PBS, store at 4°C. 13. HEPES Buffer Solution (1 M; Invitrogen, cat. no. 15630-080) (100 mL). 2 Lentiviral Transduction of Human HSCs and Progenitor Cells 1. Highly transfectable cell line to be used as packaging cells. This protocol describes conditions optimized for 293T cells. Imaging Cell Fates and Function 27 2. Growth media for packaging cells. For 293T: DMEM with 10% heat inactivated fetal bovine serum, 100 U/mL penicillin and 100 U/mL streptomycin, 2 mM Lglutamine, 50 µg/mL 2-mercaptoethanol.
Blood 102, 3478–3482. Cunningham, C. , Yang, P. , McConnell, M. , Pauly, J. , and Conolly, S. M. (2005) Positive contrast magnetic resonance imaging of cells labeled with magnetic nanoparticles. Magn. Reson. Med. 53, 999–1005. Kim, Y. , Gambhir, S. , and Witte, O. N. (2004) Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene. Mol. Imaging Biol. 6, 331–340. , and Germain, R. N. (2002) Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue.
Neurosci. Res. 76, 232–243. , et al. (2002) Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging. Neuroimage 17, 803–811. , et al. 6 T) magnetic resonance imaging. Neuroimage 24, 635–645. , et al. (2003) Dynamic tracking of human hematopoietic stem cell engraftment using in vivo bioluminescence imaging. Blood 102, 3478–3482. Cunningham, C. , Yang, P. , McConnell, M. , Pauly, J. , and Conolly, S. M. (2005) Positive contrast magnetic resonance imaging of cells labeled with magnetic nanoparticles.