Development of Sea Urchins, Ascidians, and Other by Charles E. Ettensohn, Gary M. Wessel, Gregory Wray

By Charles E. Ettensohn, Gary M. Wessel, Gregory Wray

This publication offers a realistic consultant to experimental equipment for learning the improvement invertebrate deuterostomes as animal version platforms. The chapters offer precise experimental protocols that hide a huge variety of issues in glossy experimental equipment. issues coated variety from rearing embryos to the care of grownup animals, whereas additionally providing the fundamental experimental equipment together with gentle and electron microscopy, used to review gene expression, transgenics, opposite genetics, and genomic ways. * Covers quite a lot of tools, from classical embryology via glossy genomics* Discusses animals with regards to vertebrates, delivering a useful evolutionary standpoint* contains a useful consultant to using sea urchins within the instructing laboratory

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Extra info for Development of Sea Urchins, Ascidians, and Other Invertebrate Deuterostomes: Experimental Approaches

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Garstang, W. J. (1928). The morphology of the Tunicata, and its bearing on the phylogeny of the Chordata. Quart. J. Micros. Sci. 72, 51–187. Gee, H. (1996). ’’ Chapman and Hall, London, UK. Gross, J. , and McClay, D. R. (2001). The role of Brachyury (T) during gastrulation movements in the sea urchin Lytechinus variegatus. Dev. Biol. 239, 132–147. , and Satoh, N. (2002). Conserved expression pattern of BMP-2/4 in hemichordate acorn worm and echinoderm sea cucumber embryos. Zoolog. Sci. 19, 1113–1121.

The potency of the first two cleavage cells in echinoderm development. Experimental production of partial and double formations. In ‘‘Foundations of Experimental Embryology’’ (B. H. Willier and J. M. ). Hafner, New York, 1974. , and Lepage, T. (2004). Nodal and BMP2/4 signaling organizes the oral–aboral axis of the sea urchin embryo. Dev. Cell. 6, 397–410. Durand, D. (2003). Vertebrate evolution: Doubling and shuZing with a full deck. Trends Genet. 19, 2–5. Eaves, A. , and Palmer, A. R. (2003).

Trends Genet. 17, 237–239. , Cameron, R. , Davidson, E. , and Ettensohn, C. A. (2001). A large-scale analysis of mRNAs expressed by primary mesenchyme cells of the sea urchin embryo. Development 128, 2615–2627. This Page Intentionally Left Blank PART I Procurement, Maintenance & Culture of Oocytes, Embryos, Larvae, and Adults This Page Intentionally Left Blank CHAPTER 2 Care and Maintenance of Adult Echinoderms S. Anne Bo¨ttger,* Charles W. Walker,* and Tatsuya Unuma{ *Department of Zoology and Marine Biology Biomedical Research Group Durham, New Hampshire 03824 { National Research Institute of Aquaculture Nansei, Mie 516-0193 I.

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