Differentiation of Embryonic Stem Cells by Author Unknown

By Author Unknown

This quantity covers all facets of embryonic stem mobile differentiation, together with mouse embryonic stem cells, mouse embryonic germ cells, monkey and human embryonic stem cells, and gene discovery. * Early dedication steps and new release of chimeric mice* Differentiation to mesoderm derivatives* Gene discovery through manipulation of mouse embryonic stem cells

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Quinones and Quinone Enzymes (Part B) (in preparation) Edited by HELMUT SIES AND LESTER PACKER Section I Differentiation of Mouse Embryonic Stem Cells This Page Intentionally Left Blank [1] Lineage Specific Differentiation of Mouse ES Cells: Formation and Differentiation of Early Primitive Ectoderm-like (EPL) Cells By JOY RATHJEN and PETER D. 1 There is particular merit in differentiation regimes that recapitulate lineage establishment during normal embryogenesis. These are anticipated to provide a nontransformed model system for the investigation of cell fate choice, identification, characterization and production of transient differentiation intermediates, and identification of signaling pathways that regulate cell identity and acquisition of positional information.

Sufficient EPLEBs are collected for several in situ hybridization experiments, usually 10–20 ml/timepoint. Aggregates are pelleted 23 B. G. Herrmann, Expression pattern of the Brachyury gene in whole-mount Twis/Twis mutant embryos, Development 113, 913–917 (1991). 24 L. H. Pevny, S. Sockanathan, M. Placzek, and R. Lovell-Badge, A role for SOX1 in neural determination, Development 125, 1967–1978 (1998). 25 M. Blum, S. J. Gaunt, K. W. Y. Cho, H. Steinbeisser, B. Blumberg, D. Bittner, and E. M. De Robertis, Gastrulation in the mouse: the role of the homeobox gene goosecoid, Cell 69, 1097–1106 (1992).

365 Copyright ß 2003, Elsevier Inc. All rights reserved. 00 4 DIFFERENTIATION OF MOUSE ES CELLS [1] differentiate into cells representative of the primary germ layers, ectoderm, endoderm, and mesoderm. Further differentiation results in formation of many of the cell populations of the embryo and adult including beating cardiomyocytes, blood, hepatocytes, neurons, epidermis, and gut endothelium. The major difference from development in vivo is the lack of organizational cues associated with the body axes.

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